Buy BPC-157 (Body Protection Compound-157) is a pentadecapeptide made up of 15 amino acids. The amino acids sequence in BPC-157 is similar to a portion of the human BPC amino acid sequence. Human BPC is found in the gastric juice. Experiments have shown that BPC-157 enhances the healing of wounds, including tendons wounds such as transected Achilles tendons of rats. The aim of this study was to investigate the probable mechanism that BPC-157 utilizes to accelerate the healing process in an injured tendon. The study used two group of tendon explants of which one group was cultured in a BPC-157 containing medium while the other group was cultured in a medium lacking BPC-157. These cultures were thereafter examined for tendon fibroblasts outgrowths. Such outgrowths indicated tendon regeneration.The results revealed that the explants’ outgrowth was significantly accelerated in the culture containing BPC-157 as compared to the culture lacking BPC-157. Also, a MTT assay did show that BPC-157 does not directly affect cellular proliferation in a culture of rat-derived Achilles tendon. However, results also showed that BPC-157 significantly increased the survival of cells under oxidative stress. Furthermore, the Transwell filter migration assay showed that BPC-157 significantly increased in-vitro fibroblast migration in a dose-dependent fashion. Moreover, BPC-157 accelerated the dispersal of the fibroblasts in culture dishes in a dose-dependent manner.
Additionally, FITC-phalloidin staining was able to demonstrate that BPC-157 induces F-actin formation in fibroblasts. Likewise, Western blot analysis was able to detect the production and activation of paxillin and FAK proteins. The western blot analysis also showed that BPC-157 increases the extent of phosphorylation of paxillin and FAK proteins without affecting the amounts produced.
Thus, it can be concluded that BPC-157 enhances the ex-vivo growth and in-vitro cellular migration of fibroblasts derived from rat tendon explants. Moreover, BPC-157 also increases the probability of a cell surviving under oxidative stress. These actions of BPC-157 are probably mediated by the activation (through phosphorylation) of the proteinic FAK-paxillin pathway.
BPC, for reasons you’re about to discover, stands for “Body Protecting Compound”. Your body already makes it in your own gastric juices in very small amounts, where it serves to protect and heal your gut. But if you can get the super concentrated version and get it into your system, it has an extremely high level of biological healing activity just about anywhere you put it And if many of the amino acids above look familiar to you, there’s good reason. I’ve talked about them before. I’ve used them orally for quite some time to heal injuries more quickly, to keep the body in anabolic state during or post-exercise, and to stave off central nervous system fatigue during long bouts of exercise.
What Does BPC-157 Do?
BPC-157 is surprisingly free of side effects, and has been shown in research that’s been happening since 1991 to repair tendon, muscle, intestines, teeth, bone and more, both in in-vitro laboratory “test-tube” studies, in in-vivo human and rodent studies, and when used orally or inject subcutaneously (under your skin) or intramuscularly (into your muscle).
Just take a look at the following, all of which was hunted down and identified by the good folks at Suppversity in their article on BPC-157. BPC-157 has been shown to:
-Promote tendon and ligament healing by tendon outgrowth, cell survival, and cell migration in a rodent model of Achilles tendon rupture, and also when administered in drinking water to rats with damaged medial collateral ligaments
-Tendon-to-bone healing effective enough that they may actually “successfully exchange the present reconstructive surgical methods”
-Counter the damaging effects of NSAIDs like ibuprofen or advil on the gut lining so effectively that scientists termed BPC-157 “a NSAIDs antidote” one of which they say that “no other single agent has portrayed a similar array of effects“
-Repair the damage from inflammatory bowel disease (IBD) within just days of oral administration in a rodent model of IBD
-Help cure perdidontitis when administered in a rodent model of periodontitis, significantly enough to have scientists conclude that “BPC-157 may represent a new peptide candidate in the treatment of periodontal disease“
-Reverse systemic corticosteroid-impaired muscle healing, in a rodent models where BPC-157 was administered orally once daily for 14 days to rats with crushed gastrocnemius muscle. Similar benefits were demonstrated in a rodent study by Novinscak et al.
-Accelerate bone healing in rabbits who suffered segmental bone defect before being treated with BPC-157 .
BPC-157 is also known as a “stable gastric pentadecapeptide”, primarily because it is stable in human gastric juice, can cause an anabolic healing effect in both the upper and lower GI tract, has an antiulcer effect, and produces a therapeutic effect on inflammatory bowel disease (IBD) – all again surprisingly free of side effects.
As demonstrated in the research studies cited above, BPC-157 also accelerates wound healing, and, via interaction with the Nitric Oxide (NO) system, causes protection of endothelial tissue and an “angiogenic” (blood vessel building) wound healing effect. This occurs even in severely impaired conditions, such as in advanced and poorly controlled irritable bowel disease, in which it stimulates expression of genes responsible for cytokine and growth factor generation and also extracellular matrix (collagen) formation, along with intestinal anastomosis healing, reversal of short bowel syndrome and fistula healing – all of which can extremely frustrating issues in people who have gut pain, constipation, diarrhea and bowel inflammation.
So if you have frustrating joint pain that won’t go away, some kind of muscle tear, sprain or strain, or gut “issues”, you should definitely keep reading.
This is where the bacteriostatic water (also known as “BAC water”) comes in. Here’s what to do with the BPC-157 powder and BAC water: Pop the caps off both the BPC-157 and BAC. Gently alcohol swab the rubber stopper on the BPC-157, then let it dry. Same goes with the BAC vial. Dose out the correct amount of BAC. In the case of a 30ml bottle of BAC, if you fill three insulin syringes full of water, then very slowly and carefully (peptides are extremely fragile) inject each of those syringes into a 5mg bottle of BPC-157, you are going to nearly completely fill the 5mg bottle of BPC-157. Once the 5mg bottle of BPC-157 is full, then based on this very handy Peptide Mixing & Dosing Calculator, each time you inject a 1ml/1cc syringe into it and pull that syringe back to the fifteenth tick mark, you are going to have yourself approximately 250mcg of BPC-157. Voila. You’re done. You now have a bottle of completely reconstituted and ready-to-rumble BPC-157.
Before and after reconstitution, keep your BPC-157 away from UV rays, sunlight and heat. It will remain stable at room temperature for up to ten weeks, but for best storage and results, you should store in your refrigerator, in which case it will stay stable for up to six months. It will stay stable in a freezer for up to two years. The stuff is super fragile. I cannot emphasize enough the importance of very slow and patient reconstitution. When you spray the BAC into the BPC, aim for the sides of the BPC bottle, and don’t spray directly onto the BPC powder itself. When you draw the BPC up into the syringe for injection or oral use, turn the bottle of BPC upside down very carefully and then draw the liquid up into the syringe very slowly. When you inject it or spray it into your mouth, inject it very slowly. If you keep it in the refrigerator, keep it away from things that might knock it over or jostle it around. You get the idea. Treat your BPC like the fragile, million-dollar healing elixir that it is.
How Much BPC-157
To Take There is an abundance of research on BPC-157 and it has been shown to be effective systematically when injected once daily at anywhere from 1-10mcg per kg of body weight. In most cases, this comes out to a dose of anywhere from 200mcg up to 800mcg. Some folks report the most success dosing twice per day with 250-350mcg for a total of 500-700mcg per day. So as you can see, there’s quite a bit of variability in dosage recommendations. I initially began to use BPC-157 for golfer’s elbow (inner elbow pain also known as “climber’s elbow” or “medial epicondylitis), and I personally, based on the majority of the research studies in humans, settled upon a self-administered subcutaneous BPC-157 injection of 250mcg in my left elbow on one day, then 250mcg in my right elbow the next day, for a total of two weeks. At this point, my elbow pain had completely disappeared, so I stopped. Although, as I’ve mentioned, BPC-157 is free of side effects at normal dosages, I can tell you that the first time I used it, I accidentally dosed with 2500mcg instead of the 250mcg I planned to initially trial with, and I felt for about the next four hours what I would best describe as a slight amount of irritability and grumpiness, which was likely due to the anabolic cortisol response from a very minor overdose. This feeling passed within four hours, and I’ve felt no ill-effects since with daily dosing of 250mcg.
How Long To Take BPC-157
Like I mentioned above, my bilateral inner elbow pain completely disappeared after I self-injected subcutaneously with 250mcg BPC-157 on alternating days to either the left elbow or right elbow for a total of two weeks. After the elbow pain was gone, I strained my right hamstring while hill sprinting, and began daily intramuscular “lower-butt” injections of BPC-157 for a total of ten days, at which point, my hamstring was completely healed and pain-free. Finally, for the pure joy of oral self-experimentation, I sprayed from a syringe 250mcg of BPC-157 into my mouth for a total of seven days. The BPC-157 induced a very similar feeling to the feeling I experience when drinking a cup of bone broth or eating a bowl of soaked chia seeds: a feeling of the GI tract being “coated” with some kind of protection, and a complete lack of any digestive issues, gas or bloating following any meals during that week-long time frame. I realize that’s a bit of a woo-woo explanation, and didn’t actually involve me gut testing for inflammatory markers like calmodulin or lactoferrin, but from an N=1 standpoint, it certainly seemed to me as a quite viable option for anyone who struggles with irritable bowel issues or gut inflammation. Based on the current human studies to date, BPC-157 can be safely administered for four weeks, followed by a two week rest. Speaking from my own personal experience, in which pain subsided after no more than two weeks of injections, I wouldn’t imagine you’d need to repeat this cycle unless you re-injured yourself.